Reporter
mRFP1-NLS

Part:BBa_K678034:Design

Designed by: DTU-Denmark-2   Group: iGEM11_DTU-Denmark-2   (2011-09-10)

mRFP1-NLS, targeted to the nucleus


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 345
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 345
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 345
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 345
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was designed for the Plug'n'Play with DNA assembly standard (RFC 80), therefore this part contains tails at the 5' and 3' ends as shown on the figure below. Please notice that mRFP1-NLS is not the same as BBa_K678030, BBa_K678031, or BBa_K678032.


Source

From p71-mRFP1 of the Center for Microbial Biotechnology plasmid collection at the Technical University of Denmark. The NLS sequence was synthesized.

References

(1) Campbell RE, Tour O, Palmer AE, Steinbach PA, Baird BS, Zacharias DA, Tsien RY (2002) A monomeric red fluorescent protein. Proc Natl Acad Sci USA 99:7877–7882.

(2) Toews MW, Warmbold J, Konzack S, Rischitor P, Vienken DVK, Vinuesa C, Fischer HWR (2004) Establishment of mRFP1 as a fluorescent marker in Aspergillus nidulans and construction of expression vectors for high-throughput protein tagging using recombination in vitro (GATEWAY). Curr Genet 45: 383–389.